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PMID: 27686523 [Indexed for MEDLINE] Publication Types: Comparative Study; Evaluation Studies; Validation Studies; MeSH terms gamma-H2AX Pharmacodynamic Assay Kit. The gamma-H2AX Pharmacodynamic Assay Kit is for the study of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies. Contract Services are also available. 2013-11-29 Gamma-H2AX assay is an immuno-fluorescence experiment that enables detecting the location and number of DNA double strand breaks (DSBs) in cells. Assuming that a single gamma-H2AX focus corresponds to a single DSB, one can convey the severity of cellular damage by the number of gamma-H2AX foci per cell (FPC) [1].

Gamma h2ax assay

The prognostic value of gamma-H2AX is indicated in certain cancer types, such as breast or endometrial cancer, but further investigation is needed to establish gamma-H2AX as a prognostic marker. Gamma-H2AX foci detection is the standard method to quantify DNA double-strand break (DSB) induction and repair. In this study, we investigated the induction and decay of γ-H2AX foci of different tumor cell lines and fibroblasts with known mutations in DNA damage repair genes, including ATM, LigIV, DNA-PKcs, Rad51 and Rad54. A radiation dose of 2.4 Gy was used for either an acute single high We quantified gamma-H2AX foci manually and automatically.

2016-03-04 · The gammaH2AX assay for genotoxic and nongenotoxic agents: comparison of H2AX phosphorylation with cell death response. Toxicol Sci 140, 103–117 (2014). CAS Article Google Scholar A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry.

Utveckling Av En Y-H2AX-analys Med Hög Genomströmning

Under uniform radiation hi azeez, i use software to count the gamma H2AX foci. for this olympus fluoview microscope helps a lot. to count the foci please follow the steps. set an ROI in one of the foci inside cell

‪Francesca Cappellini‬ - ‪Google Scholar‬

Additional synonyms or past names for this protein may include: H2A.X, H2A/X, H2AX. 2013-11-29 · Additionally, although our data clearly indicate a non-cumulative response of the γ-H2AX phosphorylation and stress the impact of split dose exposures on the DNA repair machinery, the clinical relevance of these findings will have to be validated for the specific cell lines and 3D/in-vivo models in which the γ-H2AX assay is proposed as a biomarker.

2010; Ivey et al. 2009). Invitrogen Anti-Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal (RM224), Catalog # MA5-33062. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This antibody reacts with All, Human, Mouse, Rat samples.
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The γ-H2AX assay has been applied for a variety of human tissues and cells.

H2AX hat auch medizinische Bedeutung als Laborwert für DNA-Schäden. injectors for kinetic assays. The instrument was used to image the stained DNA using the GFP imaging channel. Integrated Gen5™ Microplate Reader and Imager Software controls Cytation 5, and also automates image capture, analysis and processing.
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This ap-proach allows for radiation dose estimation from a mea-surement of cH2AX foci, as previously demonstrated in human cells and non-human primates (Redon et al. 2010; Ivey et al. 2009).

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This modification, called gamma-H2AX (phospho-Ser139), triggers cell cycle arrest and DNA damage response repair, which makes this assay particularly appropriate for studying genome stability, cell cycle, DNA repair, and more broadly as a biomarker for cancer and for aging process investigations. We quantified gamma-H2AX foci manually and automatically. In addition, total gamma-H2AX activation was determined by flow cytometry. For all chemicals the cytotoxic dose response was assayed by a metabolic cytotoxicity assay.

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This ap-proach allows for radiation dose estimation from a mea-surement of cH2AX foci, as previously demonstrated in human cells and non-human primates (Redon et al.

In the detection of genomic damage, gamma-H2AX is especially important in research involving cancer treatment and therapy, and environmental biomonitoring. munocytoﬂuorescence assay can be used to quantify ra-diation-induced changes in the phosphorylation status of the histone 2A.X (H2AX) (Redon et al. 2009). This ap-proach allows for radiation dose estimation from a mea-surement of cH2AX foci, as previously demonstrated in human cells and non-human primates (Redon et al.